Part:BBa_I716210:Design

BamHI endonuclease without start codon

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites.

PCR sil04F and sil04R on BamHI methylase/endonuclease region. (657 bp, BglII/XhoI) Sub into pBca9145-Bca1089 (BglII/XhoI,2063+696, L) Product is pBca9145-I716210

sil04F Forward BglII biobrick of BamHI ccaaaAGATCTgaagtagaaaaagagtttattactga sil04R Reverse XholI/BamHI biobrick of BamHI gctagCTCGAGttaGGATCCctatttgttttcaactttatc

Source

PCR sil04F and sil04R on BamHI methylase/endonuclease region. (657 bp, BglII/XhoI) Sub into pBca9145-Bca1089 (BglII/XhoI,2063+696, L) Product is pBca9145-I716210